Protective effect of vitamin E [alpha tocopherol acetate] on diclofenac-induced nephrotoxicity in young albino rats

To determine the preventive role of Vitamin E on renal parenchyma after given of Diclofenac Sodium in young albino rats. Experimental study. This study was carried out in the Department of Anatomy Baqai Medical University and Muhammad Medical College. Mirpurkhas from June 2011 to November 2011. 30 young albino rats were taken. They were divided into three groups: A, B and C. The animals in group-A given normal saline 10 ml/kg per day. Group-13 received diclofenac sodium 2 mg/kg per day and group-C receives diclofenac sodium 2mg/kg/day dissolved in distilled water with vitamin-E 2 mg/kg/day dissolved in olive oil administered half an hour before the diclofenac sodium by feeding tube per day for 2 weeks. On day 15 all animals were sacrificed with deep ether anesthesia. Their kidneys were removed, fixed in 10% formalin, Representative blocks were taken and embedded in liquid paraffin. For routine histological examination 5 pm thick section cut by niicrotome and stained with HandE, PAS and silver methenamine Renal histology was done under- light microscope to see the proximal and distal tubular diameter and count. No significant [PT0.05] changes were observed in the histopatholov of kidney tissues of the groups A and C rats. The group B significantly [P<0.001] affected the histopathology of kidney. It may be concluded that diciofenac sodium produces changes in kidne, which may be attributed to ischaemia induced by inhibition of prostaglandin synthesis resulting in tubular necrosis in young albino rats simultaneous administration of vitamin-E partially protect the morphological and histological changes induced by diclofenac sodium

Effects of gentamicin on renal parenchyma and prevention by vitamin E in young albino rats

To determine the preventive role of Vitamin E on renal parenchyma after given of gentamicin in young albino rats. Experimental study. This study was carried out in the Department of Anatomy Baqai Medical University and Muhammad Medical College, Mirpurkhas from June 2011 to November 2011. 30 young albino rats were taken. They were divided into three groups ; A, B and C. The animals in group-A given normal saline 10 ml/kg/day intraperitoneal for 2 weeks. Group-B received gentamicin 100 mg/kg/day intraperitoneal for 2 weeks and group-C receives gentamicin 100mg/kg/day intraperitoneal with vitamin-E 2 mg/kg/day orally for 2 weeks. On day 15 all animals were sacrificed with deep ether anesthesia. Their kidneys were removed, fixed in 10 % formalin. Representative blocks were taken and embedded in liquid paraffin. For routine histological examination 5 microm thick section cut by microtome and stained with H and E, PAS and silver methenamine. Renal histology was done under light microscope to see the proximal and distal tubular diameter and count. No significant [P>0.05] changes were observed in the histopathology of kidney tissues of the groups A and C rats. The group B significantly [P<0.001] affected the histopathology of kidney. It may be concluded that gentamicin produces changes in kidney, which may be attributed to ischaemia resulting in tubular necrosis in young albino rats simultaneous administration of vitamin-E partially protect the morphological and histological changes induced by gentamicin

Protective effect of vitamin E [a tocopherol acetate] on gentamicin-induced nephrotoxicity in adult albino rats

To determine the preventive role of Vitamin E on renal parenchyma after given of gentamicin in adult albino rats. Experimental study. This study was carried out in the Department of Anatomy Baqai Medical University and Muhammad Medical College, Mirpurkhas from June 2011 to November 2011. For this study, 30 adult albino rats were taken. They were divided into three groups ; A, B and C. The animals in group-A given normal saline 10 ml/kg/day intraperitoneal for 2 weeks. Group-B received gentamicin 100 mg/kg/day intraperitoneal for 2 weeks and group-C receives gentamicin 100mg/kg/day intraperitoneal with vitamin-E 2 mg/kg/day orally for 2 weeks. On day 15 all animals were sacrificed with deep ether anesthesia. Their kidneys were removed, fixed in 10% formalin. Representative blocks were taken and embedded in liquid paraffin. For routine histological examination 5 micro m thick section cut by microtome and stained with H and E, PAS and silver methenamine. Renal histology was done under light microscope to see the proximal and distal tubular diameter and count. No significant [P>0.05] changes were observed in the histopathology of kidney tissues of the groups A and C rats. The group B significantly [P<0.001] affected the histopathology of kidney. It may be concluded that gentamicin produces changes in kidney, which may be attributed to ischaemia resulting in tubular necrosis in adult albino rats simultaneous administration of vitamin-E partially protect the morphological and histological changes induced by gentamicin

Effects of diclofenac sodium on renal parenchyma and prevention by vitamin-E in adult albino rats

To determine the preventive role of Vitamin E on renal parenchyma after given of Diclofenac Sodium in albino rats. Experimental Study This study was conducted in the Department of Anatomy Baqai Medical University and Muhammad Medical College, Mirpurkhas from June 2011 to November 2011, For this experimental study, 30 albino rats were taken. They were divided into three groups ; A, B and C. The animals in group-A given normal saline 10 ml/kg per day. Group-B received diclofenac sodium 2 mg/kg per day and group-C receives diclofenac sodium 2mg/kg/day dissolved in distilled water with vitamin-E 2 mg/kg/day dissolved in olive oil administered half an hour before the diclofenac sodium by feeding tube per day for 2 weeks. On day 15 all animals were sacrificed with deep ether anesthesia. Their kidneys were removed, fixed in 10% formalin. Representative blocks were taken and embedded in liquid paraffin. For routine histological examination 5 micro m thick section cut by microtome and stained with H and E, PAS and silver methenamine. Renal histology was done under light microscope to see the proximal and distal tubular diameter and count. No significant [P>0.05] changes were observed in the histopathology of kidney tissues of the groups A and C rats. The group B significantly [P<0.001] affected the histopathology of kidney. It may be concluded that diclofenac sodium produces changes in kidney, which may be attributed to ischaemia induced by inhibition of prostaglandin synthesis resulting in tubular necrosis in albino rats simultaneous administration of vitamin-E partially protect the morphological and histological changes induced by diclofenac sodium

Toxicity of isoniazid [INH]; role of biochemical monitoring tests and history of weakness. the management of pulmonary tuberculosis in placebo controlled study

To compare the untoward effects of isoniazid [inh] and placebo as an anti-tuberculosis therapy in tuberculosis patients. Study Prospective study. Free T.B Clinic, Muhammad Medical College Hospital Mirpurkhas Sindh. Period: June 2007 to Dec 2007. Patients, Method and Patients were selected with filling of consent forms from free T.B Clinic. Prospective study of adverse effects of INH Isoniazid in tuberculosis treatment. It is concluded like other anti-biotics, Isoniazid must be carefully, mentioned in combination therapy of Anti-Tuberculosis Therapy. The major side effects are those giving rise to serious health hazards, require discontinuation of the drug and referral to chest physician